“How long read sequencing on the iSeq 100 system enables 16S rRNA analysis”
The Illumina iSeq 100 Sequencing System is a small footprint benchtop NGS instrument that offers big advantages. The system provides fast and efficient microbial sequencing through a streamlined, comprehensive workflow which includes library preparation, sequencing and data analysis.
This workshop will provide an Illumina technology update, a technical introduction to the iSeq100 sequencer followed by a presentation from the ChunLab in Korea outlining in detail how they sequenced 16S rRNA using the upcoming 2x 250 cycle kit, with their bioinformatics solutions.
"Metagenomics for clinical and public health microbiology: real life applications"
Health Microbiology reference laboratories fulfil a critical role in providing overarching testing and surveillance for notifiable, emerging and important pathogens. As the state reference laboratory for Queensland, we have been focusing on establishing deep sequencing methods for two different purposes: 1) Hypothesis-free diagnostics, enabling detection of pathogens directly from clinical samples in confounding cases, and 2) molecular epidemiological surveillance, by generating genotyping information directly from clinical samples to fill the gap in epidemiological data caused by the increase in culture independent diagnostic testing in pathology laboratories. This presentation will discuss our experiences with the use of deep sequencing for public health microbiology and the challenges involved in implementing this technology.
Dr Rikki Graham, Snr Scientist Public Health Microbiology, Forensic and Scientific Services, Queensland Health
Please join us during the lunch break on Monday 1 July for an interesting workshop on antimicrobial susceptibility testing. The session will cover the calibration of ceftolozane-tazobactam discs for use with the CDS test, polymyxin B disc testing and the use of carbapenems in disc diffusion. The workshop is open to all attendees with an interest in phenotypic susceptibility testing.