Monday, 1st July Australian Society for Microbiology Annual Scientific Meeting 2019

• Microbes that have changed the world: Influenza in the vulnerable elderly - Richard I Lindley
• HIV: still a global problem requiring prevention and cure - Anthony Cunningham
• Not available - Dominic Dwyer
• characterisation of influenza viruses circulating in Australia during a high inter-seasonal period in 2018-9 - Heidi Peck
• Role of IRGM polymorphism in Helicobacter pylori related Gastric cancer. - Apeksha B Goswami
• Remodeling of pSK1 Family Plasmids and Enhanced Chlorhexidine Tolerance in Methicillin-Resistant Staphylococcus aureus - Sarah L Baines

• Progress towards a gonococcal vaccine - Kate L Seib
• Rapid Response Pipeline for Stabilized Subunit Vaccines - Keith Chappell
• Developing Attenuated Live Vaccines to Control Mycoplasmoses in Livestock - Glenn F Browning
• Direct interaction of whole inactivated influenza A and pneumococcal vaccines enhances influenza-specific immunity - Mohammed Alsharifi
• The Neisseria gonorrhoeae Methionine Sulfoxide Reductase (MsrA/B) Is a Surface Exposed, Immunogenic, Vaccine Candidate - Freda Jen
• Zika Virus Non-structural Proteins as DNA Vaccine Antigens to Elicit Cell Mediated Immunity - Ashish C Shrestha

• The “antenatal microbiome”: controversies, complications and consequences - Jeff Keelan
• How does a long-term Paleolithic diet affect our gut health and markers of cardiovascular disease risk? - Claus T Christophersen
• What do we mean by dysbiosis and how do we manage it? - Andrew Holmes
• Cecal luminal microbial diversity profiling of laying chicks is manipulated by probiotics supplementation and Salmonella Typhimurium challenge - Samiullah Khan
• Host nutrition manipulation for gut microbiota modulation - Alison WS Luk
• The Antimicrobial side of Antidepressants. A cause for Anxiety? - Gal Winter

• The chitin synthase concert: orchestration of chitin synthesis at septation sites in Candida albicans - Megan D Lenardon
• Latent Toxoplasma relies on effector proteins to prevent IFNg-mediated cell death - Christopher J. Tonkin
• Dissecting the dual functionality of the Tox3 effector protein from the wheat pathogen Parastagonospora nodorum - Peter S Solomon
• Parasites of land and sea: Comparative genomics of microbial eukaryotes Perkinsus olseni and Theileria orientalis. - Daniel R Bogema
• Potential applications of vinasse as low-cost culture media for fungi - Sandhya K Jayasekara
• Chagas disease in Australia: What are the risks? - Robert L Flower

• Inflammation and the Microbiome:A Dangerous Liaison - Alan Landay

• Current and Future Trends in Diagnostic Microbiology - Karen Carroll

“How long read sequencing on the iSeq 100 system enables 16S rRNA analysis”

The Illumina iSeq 100 Sequencing System is a small footprint benchtop NGS instrument that offers big advantages. The system provides fast and efficient microbial sequencing through a streamlined, comprehensive workflow which includes library preparation, sequencing and data analysis.

This workshop will provide an Illumina technology update, a technical introduction to the iSeq100 sequencer followed by a presentation from the ChunLab in Korea outlining in detail how they sequenced 16S rRNA using the upcoming 2x 250 cycle kit, with their bioinformatics solutions.

"Metagenomics for clinical and public health microbiology: real life applications"

Health Microbiology reference laboratories fulfil a critical role in providing overarching testing and surveillance for notifiable, emerging and important pathogens. As the state reference laboratory for Queensland, we have been focusing on establishing deep sequencing methods for two different purposes: 1) Hypothesis-free diagnostics, enabling detection of pathogens directly from clinical samples in confounding cases, and 2) molecular epidemiological surveillance, by generating genotyping information directly from clinical samples to fill the gap in epidemiological data caused by the increase in culture independent diagnostic testing in pathology laboratories. This presentation will discuss our experiences with the use of deep sequencing for public health microbiology and the challenges involved in implementing this technology.

Dr Rikki Graham, Snr Scientist Public Health Microbiology, Forensic and Scientific Services, Queensland Health

Please join us during the lunch break on Monday 1 July for an interesting workshop on antimicrobial susceptibility testing. The session will cover the calibration of ceftolozane-tazobactam discs for use with the CDS test, polymyxin B disc testing and the use of carbapenems in disc diffusion. The workshop is open to all attendees with an interest in phenotypic susceptibility testing.

• Exploiting the struggle for haem:  a new respiratory probiotic candidate? - Brianna Atto
• A multi-component toxin from Bacillus cereus activates the NLRP3 inflammasome - Anukriti Mathur
• The not-so-sterile womb: Evidence that the human fetus is exposed to bacteria prior to birth - Lisa Stinson
• Between a rock and a hard place: Acinetobacter baumannii loses virulence and antibiotic resistance when escaping killing by lytic phages. - Fernando Gordillo Altamirano
• Nitroxides: a promising therapeutic strategy for the treatment of Staphylococcus aureus biofilm-related infections. - Anthony D Verderosa
• Exploring merozoite surface protein 2 function and vaccine potential during malaria red blood cell invasion. - Isabelle Henshall

• Nanostructured antibacterial surfaces - Krasimir Vasilev
• Development of antimicrobial contact lenses - Mark Willcox
• The clinical relevance of anti-biofilm coated devices against Candida species - Carla Giles
• Liquid Metal Particles Used to Treat Pathogenic Mature Biofilms via Magneto-Physical Activation - Vi Khanh Truong
• Antibiofilm activities of pentacyclic triterpenoids against methicillin-resistant and biofilm-forming Staphylococcus aureus (MRSA) - Pooi Yin Katrina Chung
• Strategies for Combatting the Formation of Fungal Biofilms on Biomaterials Surfaces - Javad Naderi

• Identification of estrogen receptor modulators as inhibitors of flavivirus infection - Nicholas Eyre
• Not available - Nicholas Shackel
• Structural and non-structural roles of the matrix protein of respiratory syncytial virus - Reena Ghildyal
• Discovery of novel crustacean and cephalopod flaviviruses: insights into evolution and circulation of flaviviruses between marine invertebrate and vertebrate hosts - Rhys Parry
• The role of viperin in dengue virus infection - Jillian M Carr
• CRISPR/Cas9 genome-wide KO screen reveals RACK1 as a critical pan-flavivirus host factor for virus replication - Byron Shue

• The Center for Infectious Disease Research and Policy: Antimicrobial Stewardship Project - Marnie Peterson
• Not available - Stephen Page
• The National Centre for Antimicrobial Stewardship – A One Health Approach - Caroline Chen
• Capsule-switching is associated with the recent global expansion of the fluoroquinolone-resistant Escherichia coli sequence type (ST)1193 clone - Rhys T. White
• Rapid respiratory pathogen testing: assessing test appropriateness and impact on antimicrobial prescribing - Thean Yen Tan
• Induction of Erythromycin resistance in Bordetella holmesii confirmed by whole genome sequencing - Winkie Fong

• Weird and wacky foods and public health - Vanessa McEvoy
• Detecting hospital transmission of superbugs using genomics - Claire Gorrie
• Collateral Damage: Biocide Use and the Co-Selection of Multidrug Resistant Staphylococcus aureus - Glen P Carter
• Laboratory-based surveillance of Clostridium difficile infection in the Australian healthcare and community settings in 2015 - Stacey Hong
• Microbiological monitoring in the production of exported meat - assessing hygiene using the Product Hygiene Indicators Program - Mark A Salter
• The evolutionary implications of mobile genetic element acquisition in the multidrug resistant Escherichia coli ST101 clone. - Melinda M Ashcroft

• Group A Streptococcal interactions with the host - Martina Sanderson-Smith
• Immune modulation by Helicobacter pylori and bacterial outer membrane vesicles. - Maria Liaskos
• ­Evolution of a Clade of Acinetobacter baumannii Global Clone 1, Lineage 1 via Acquisition of the oxa23 Carbapenem Resistance Gene and Dispersion of ISAba1 - Mohammad Hamidian
• Living on air: trace gases as hidden energy sources for the dormant bacterial majority - Chris Greening

• Molecular analysis of two strains of Barmah Forest virus isolated by Australian Defence Force - Wenjun Liu
• Causal factors contributing to host immune system change in koalas immunized against chlamydial infection. - David Lizarraga
• Multilevel genome typing and global epidemiology of Salmonella Enteritidis - Lijuan Luo
• Case report: whole genome sequencing based investigation of maternal-neonatal listeriosis - Lijuan Luo
• Comparative proteomics of Australian epidemic Bordetella pertussis - Laurence Don Wai Luu
• Pertactin-Negative and Filamentous Hemagglutinin-Negative Bordetella pertussis, Australia, 2013–2017 - Laurence Don Wai Luu
• SpeeDx PlexPCR RespiVirus Assay Detect a Broad Range of Contemporary Human Influenza Strains - Sandhya Nair M Rajan
• Structure and function of the Enterobacterial Common Antigen polysaccharide polymerase WzyE - Nicholas Maczuga
• Prevalence of fecal coliforms and Escherichia coli in drinking water from source and point-of-use in Rohingya camps, Bangladesh - Zahid Hayat Mahmud
• A Case of Nocardia brasiliensis in a Gardening Wound - Robekah McDougall
• Proteome analysis during fruit body development in the edible mushroom Lentinula edodes - Yasumasa Miyazaki
• LIFE PROCESSES IN OUR GROUNDWATER - Olivia Mosley
•   Bacteriophages For The Control Of 'Rattles' In Foals - Katharine Muscat
• Virulence genes rib and bca in serotypes of Group B streptococcus (GBS) isolated from symptomatic pregnant women in tertiary care hospital in East Coast Malaysia - Mohammed Imad A. MIA Mustafa Mahmud
• FUNCTIONAL METABOLIC INTERMEDIATE (2-PYRON-4,6-DICARBOXYLIC ACID) FROM LIGNIN - Masaya Nakamura
• Trans-generational effects of commensal microbiota on pupal production and body weight of a polyphagous fly - Binh Nguyen
• Identification of common Apergillus species using Matrix-Assisted Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)   - Khanh Nguyen
• Effective host depletion and microbial enrichment method for bovine genital tract microbiome research - Chian Teng CTO Ong
• Screening of bacteriophage library displaying scFv antibody fragments from schistosome-infected buffalo for diagnostic reagents for schistosomiasis - RINA B OPULENCIA
• Genomic epidemiology of erythromycin-resistant Bordetella pertussis in China - Michael Payne
• Isolation of Clostridium difficile from paediatric patients in Western Australia - Sicilia Perumalsamy
• Unravelling the three “-omes” of Pandoraea fibrosis - Miranda E. Pitt
• Molecular epidemiology of Clostridium difficile isolated from piglets in Thailand and Malaysia - Papanin Putsathit
• Calibration of Ceftolozane-Tazobactam for the CDS Method - Dianne Rafferty
• Multiplex PCR-Next Generation Sequencing for Culture-Independent Typing of Bordetella pertussis - Raisa Rafique
• Unravelling the transposition mechanism of res hunter transposon Tn502 - Vaheesan Rajabal
• Quality Characteristics of Mango Flavoured Fermented Dairy Drink - C Senaka Ranadheera
• Disrupting Bacterial Water Channels to Prevent Growth of Staphylococcus aureus - Zein Amro
• Host diversity driven phage evolution which leads to host range expansion - Duhita Sant
• Multidrug-resistant Salmonella isolated in Australia harbour a conjugative plasmid similar to that from a Salmonella strain isolated in Canada - Amreeta Sarjit
• Combination of duplex loop-mediated isothermal amplification with lateral flow biosensor assay for detection of Campylobacter jejuni and Salmonella spp. - Thanawat Sridapan
• Fishing for Neisseria gonorrhoeae –a custom amplicon panel for detection from clinical metagenome samples - Christina Straub
• Defining the mechanism of Kidney infection due to Group B streptococcus - Matthew J Sullivan
• Proteomic comparison of Australian epidemic Bordetella pertussis biofilm cells - Hiroki Suyama
• Automated Resistance Detection: Comparison of BD Phoenix to bioMérieux Vitek2 for Susceptibility Testing of Multi-drug Resistant Isolates - Patricia Szczurek
• Automated Resistance Detection: Comparison of the expert systems of BD Phoenix and bioMérieux Vitek2 for Susceptibility Testing of Multi-drug Resistant Isolates - Patricia Szczurek
• Characterization of Phase Variable Genes of Moraxella catarrhalis - . Taha
• Role of Shigella flexneri 2a serotype determinants in pathogenesis and as bacteriophage Sf6 receptors - Min Yan Teh
• Investigation of a complex resistance module in the IncM plasmid pVS20 - Steve Petrovski
• Study for Monitoring Antimicrobial Resistance Trends (SMART) in Australia and New Zealand (ANZ) in a Regional and Global Context - Merrin Tulloch
• A study of antimicrobial susceptibility pattern of Bacteroides species to amoxicillin-clavulanate, clindamycin and metronidazole from clinical isolates between 2015-2019 at Austin hospital - Rachana Viswanath
• Rapid Screen System (RSS) for Mycobacteria via Digital Pathology - Rodney McDougall
• Performance Qualification of Bruker MALDI Biotyper® 4.0 Microbial Identification System within a Regulatory Compliance Testing Laboratory - David Yeates
• Preserving proteins during microbial dormancy speeds return to growth state - Jinki Yeom
• Rapid detection and identification of common Salmonella serovars using target genes selected from comparative genomic analysis - Xiaomei Zhang
• Establishing mouse infection models with clinically relevant bacterial strains for examining Mucosal-associated invariant T (MAIT) cell immunity - Tianyuan Zhu
• Discovery & preclinical development of tetrapeptide ETC-670 for treating MRSA skin infections and nasal decolonization. - Brian CS Chia
• An evaluation study on Illumina’s iSeq 100 sequencing platform using the two commonly performed bacterial analysis. - Sung-Min Ha
• The Australian Microbiome – a collaborative network characterizing Australia’s microbial diversity - Andrew Bissett
• The Emergence of Microsporum audouinii in Australia - Lisa Panigas